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Quality Control
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Is user QC required for the tubes used to set up AFB in the Bactec MGIT 960 or is the manufacturer QC sufficient?
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Virology - general
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Is it recommendet to use swabs with AMIES transport medium for collecting influenza samples? Thank you.
iris_hatibi@hotmail.com
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Parasitology
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We want to switch from PVA to Protofix for ova and parasite collection. What validation is required? Do we need to collect samples from patients in duplicate? If so, how many positives/negatives? Is there another way to validate without involving patients?
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Mycology
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We received an identification of Saccharopolyspora species from an aortic valve. Are there any cases reporting this organism as a pathogen at this site?
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Is there an antimicrobial agent that I can add to my fungal culture plates (PDA or SDA) that will prevent bacterial growth without inhibiting fungal growth?
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We are interested in the ChromAgar Candida to give our doctors a presumptive Id on yeast for treatment. Since this a singl, presumptive ID, do we need to have a survey for this? Any help would be appreciated.
thanks
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We are currently using a modification of the hair test for dermatophytes. The hair is unsterile and plate media is SAB with chloramphenicol and getamicin (Mycology agar PML)to suppress the bacteria on the hair . I don't find this method very scientific even though it has been vlidated with appropriate organisms In your opinion is this method acceptable?
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Mycobacteriology
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mycobacteria
is it ok to heat fix afb stains at 80 degrees celcius for 15 mins?
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mycobacterium other than tuberculosis was reported by pcr in endometrial cureettage, is it significantor contaminant/ which are the significant mott in endometrium ?
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does qc need to be perform on middlebrook 7h11(nonexempt) by the user lab? also which control organisms should be run?
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Compliance
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WE DID A SKIN STREP TEST WITH A DX OF 691.0, WHAT WOULD BE THE CPT CODE? THANKS, KERRY
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Antimicrobial Testing - general
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On a few E.coli isolates, we received an interpreation of sensitive or intermediate for gentamycin and resistant for tobramycin. Should we change the interpretation for gentamycin to resistant to match the tobramycin result?
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Is there a relation between MLS(B/c)and HLR-aminoglucosides resistency in Enterococcus spp?I have observed statistical relation between these two types of resistence.Please write me on iiosifov@yahoo.com
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Bacteriology - gram postive cocci
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What is the significance of isolating MRCons from Umbical Artery Catheter & Umblical Venous Catheter of neonates.Baby is Clinically stable do we have to report it.We isolate Lot of MRCoNS from ET TIP,Trachestomy tips any guidelines on reporting.Thank you.
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Culture, blood
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Do you know if there is any reason why LMX/Lidocaine creme could not be used on a pediatric patient if blood cultures are being drawn. Would cleaning the site immediately prior to drawing the blood culture negate the effect of the lidocaine?
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Culture, stool
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Is it clinically relevant to test for C diff toxin in patients who are currently receiving either Vancomycin or Metronidazole?
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WHEN THERE IS NO GROWTH ON MEDIA PLATES,IS IT OK TO REPORT NO GROWTH ON STOOL CULTURE INSTEAD OF REPORTING NO SALMONELLA, SHIGELLA, E.COLI O157:H7 AND CAMPYLOBACTER ISOLATED?
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Culture, respiratory
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This question is in regard to Cystic fibrosis cultures. We are a small lab and have a few patients with cystic fibosis that have respiratory cultures done on a routine basis. We currently process them the same as the other respiartory cultures. At this point we have not isolated any Pseudo in the cultures. From what I read these type of cultures should have some specialized media and possibly held longer than a routine culture? Would it be appropriate for us to screen at our facility on our routine media or would we be missing an important pathogen. Any protocols or references would be appreciated.
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Culture, urine
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cystoscopic urine nephrostomy urines,how should we treat these cultures both in regard to plating and reporting.
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Culture, other
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Our CSF culture protocol includes aerobic culture, gram stain, and Cryptococcal antigen. Is there documentation for not performing repeat Cryptococcal antigens on multiple specimens? What is an acceptable time interval between repeat testing? Thanks!
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Miscellaneous
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Does HIV induce systemic lupus erytematosis? or, Does systemic lupus erytematosis cause false positive HIV results?
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